作者:刘胜雄，欧阳永忠，汤明，吴飞跃

【摘要】  目的  探讨川芎嗪（TMP）对大鼠移植肝的保护作用。方法  参照Delriviere和Kamada等方法建立大鼠肝移植模型，将大鼠随机分为3组：A组（假手术组）、B组（肝移植对照组）和C组（TMP干预组）。采用全自动生化分析仪检测术后血清肝功能(AST、ALT、LDH)水平；应用免疫组化SP法结合病理图像分析仪测定肝组织中Bcl-2、Bax的表达情况；HE染色观察其病理形态学变化。结果  （1）C组AST、ALT、LDH明显低于B组（P<0.01）。（2）C组Bcl-2或Bcl-2/Bax表达明显高于B组（P<0.05），而Bax表达无明显变化（P>0.05）。（3）A组无明显病理形态改变，B组术后肝组织出现明显的病理损害，C组病理变化明显减轻。结论  TMP可能通过上调Bcl-2或Bcl-2/Bax表达而减轻肝移植体的低温保存再灌注损伤，对供肝有保护作用。
   
    【关键词】  肝移植；保存再灌注损伤；川芎嗪；Bcl-2；Bax
   
    Protective effect of tetramethylpyrazine on liver graft in rat liver transplantation
    
    【Abstract】  Objective  To investigate the protective effect of tetramethylpyrazine (TMP) preconditioning on liver graft in rat liver transplantation.Methods  Performing the operation of liver transplantation in male Sprague-Dawley rats referred to Delriviere and Kamada with cuff anastomosing methods，the rats were randomly divided into three groups：A group(n=6，sham operation group)，B group (n=30×2，liver transplantation control group)，C group (n=30×2，TMP group).The parameters of liver function (AST，ALT and LDH) were tested by auto-biochemical analyser in group B and C after reperfusion 0.5h，3h，6h，24h，48h respectively via the plasma samples；part of the liver tissue was sampled to determine the expression of Bcl-2 and Bax.The parameters in group A were examed 3h after the operation.The expression of Bcl-2 and Bax was determined by immunohistochemisty technique with pathological imaging analyser.Also，parts of liver tissue samples were used to detect the histopathologic changes.Results  （1）The levels of AST，ALT and LDH were significantly decreased in group C compared with that in group B （P＜0.01）after reperfusion 0.5h，3h，6h，24h，48h respectively.（2）The expression of Bcl-2 were significantly increased in group C compared with that in group B （P＜0.01）after reperfusion 0.5h，3h，6h respectively.The different expression of Bax between group C and group B was nonsignificant.（3）Approximately normally morphologic structure was found in group A，some obvious histopathologic changes in group B could be seen morphologi

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